Key words
Cel- en gentechnologie
Objectives
In this course the students gain more insight into the techniques to isolate and recombinate genes and to produce genetically engineered organisms.
Topics
Theoretical course:
Restriction endonucleases, cloning vectors, recombinant DNA technology, DNA sequencing techniques, cloning DNA sequences, screening cDNA libraries and gene mapping
Practical exercises:
Construction of recombinant DNA molecules involving PCR, DNA extraction, digesting and ligating DNA, cloning experiments and agarose gelectrophoresis.
Prerequisites
Genetics, biotechnology, biochemistry and informatics
Final Objectives
Materials used
syllabus “Cel- en Gentechnologie”, lab manual, scientific publications, exercises involving word wide web searches
Study costs
10 Euro
Study guidance
Additional information can be asked
Teaching Methods
Lectures, lab exercises and project work in groups
Assessment
Theoretical course: oral examination with written preparation. Evalution of laboratory section is based on lab reports.
Lecturer(s)
Stefaan Werbrouck
Els Palmans
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