Key words Biotechnologie
Objectives
The biotechnology goes far by technical innovations towards improvements of
the environment and the circumstances of life.
Topics
The DNA molecule is the molecular basis of genetics. His complex structure
and his organisation into chromosomes is the starting-point for the study of
mutations.
The molecular basis of genetic engineering and the explanation of practical
realizations.
Sequence analysis of proteins and nucleic acids in solution or on a carrier
(automation).
Organic synthesis of proteins and nucleic acids in solution or on a carrier.
Constructions of recominants : vector, clones, genomic library
Protein modification
In the practical courses : separation of proteins, nucleic acids, plasmids.
Stability of plasmids. Use of restriction enzymes. Isolation of enzymes.
Immobilisation of cells and their use in bioreactors.
Prerequisites
Profound knowledge of enzymology, molecular biology and biochemistry.
Final Objectives
Materials used
Theoretical course : teacher's course - other textbook(s) - scientific &
technical literature - school library materials
Practical course : teacher's course
Study costs
Study guidance
Teaching Methods
Theoretical course : lectures - demonstrations - exercises
Practical course : laboratory work
Assessment
Theoretical course : oral examination
Practical course : evaluation during course - report of the results
Lecturer(s)
Raphaël ROGIERS
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