Key words
Cel- en gentechnologie, biotechnologie, recombinant
Objectives
The aim of the course is to give the student an overview of different techniques in the field of the recombinant DNA technology. Emphasis will be given to the isolation and recombination of useful genes for the transformation of organisms.
Topics
THEORETICAL:
The course gives an lineary organised overview of a number of techniques from the recombinant DNA- technology. Attention will be given to the cloning of DNA, cloning vectors for procaryotes and eucaryotes, the construction of recombinant DNA molecules, the transformation of cells, the cloning of specific genes, the selection and characterization of recombinants and different expression vectors.
PRACTICALS:
During the practicals different techniques frequently used in modern biotechnology will be covered. The excercises consist of the following items: the isolation and analysis of plasmid DNA, the isolation and cloning of restriction fragments, the construction of a recombinant DNA molecule, the selection of transformed microorganisms, the analysis of restriction fragments through agarose gelelectroforese and finally the sequencing of DNA.
Prerequisites
Knowledge of biochemistry, genetics and biotechnology
Final Objectives
· Lifelong learning
· Oral and written communication skills
· Creativity and initiative in experimental set ups
· Manage, analyse and evaluate research results and formulate conclusions
· Draw up an inventory, select and implement literature in scientific research
· Thorough knowledge of applied cell and gene technology
Materials used
Syllabus “Cell and gene technology”
Textbooks and scientific papers; websites
Study costs
Cost: 10.0 EUR
± 10 Euro
Study guidance
Additional information can always be asked
Teaching Methods
Lectures
Laboratory excercises
Assessment
Theory: Written examination with open questions
Practicals: permanent evaluation and quotation of the reports
Lecturer(s)
T.: Kathy Messens
P: Els Palmans
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