Key words
T490, B110
Objectives
Plant tissue Culture is the aseptic and controlled culture of plant parts such as isolated organs, tissues, embryos, ovaria,… on an artificial medium in a closed recipient. During the classical plant breeding process or during the vegetative propagation of a large number of crops, there is an “in vitro” phase. Also for genetic modification of a crop, knowledge about tissue culture is essential. Do we want the in vitro plant or plant tissue to produce roots, callus or new shoots? Should the plant stay short or should it grow elongated? How can we influence its senescence? This course wants to provide insight in how we control a plant or its tissue in vitro, what’s the meaning for practice and what are the problems? A number of basic skills are gained in a well-equipped laboratory. Making complex media, initiation in vitro, in vitro multiplication, acclimatization.
Topics
- Theory:
historic overview; botanical base, media, recipients, machines and their use, applications (meristem culture, callus culture, cell suspensions, protoplast culture, induction of new meristems, somatic embryogenesis, synthetic seeds, induction of haploids), problems (contamination, hyperhydricity, browning, genetic variation), lab design, commercialization.
- Exercices :
basic techniques for medium preparation, sterile manipulation, initiation by seed and axillary meristems, controlling with plant hormones. Then it’s time for own in vitro projects.
Prerequisites
knowledge about botany, organic and anorganic chemistry
Final Objectives
- Provide insight in how to control a plant or its tissue in vitro
- ability to analyze and solve practical problems in plant tissue culture
- Sense for precision and detail (exercises);
- Creativity and initiative (project work) and ability to work independently.
Materials used
Lecture notes ‘Plantenweefselteelt”, video, website
Study costs
Cost: 10.0 EUR
10 Euro
Study guidance
Permanent possibility for questioning, guidance and coaching.
Teaching Methods
Teachers courses, visit commercial plant tissue culture lab, practical exercises and demonstrations
Assessment
Oral examination with written preparation, evaluation project reports
Theory: 4/5, exercises : 1/5
Lecturer(s)
Stefaan Werbrouck
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