Key words
DNA structure, qualitatieve & quantitative genetic identification technniques, PCR, quality aspects
Objectives
The aim of the course is to give an overview of the different available genetic identification techniques. Emphasis will be given not only to the theoretical background but also several practical applications will be discussed.
Topics
THEORETICAL:
The course gives an lineary organised overview of the different available genetic identification techniques. Initially attention will be given to the basic principles for the amplification and detection of DNA and RNA. Thereafter the genetic identification techniques will be covered in detail, e.g. gelelectroforesis, restriction analysis, chromosome walking, PCR, RFLP, DNA sequencing, protein analysis. Finally an overview of some practical applications within the agro sector will be included.
PRACTICALS:
During the practical excercises different procedures will be covered for obtaining an genetic fingerprint, based on either proteins or DNA, followed by an interpretation of the obtained signals. A number of theoretical excercises will also be included in the excercises. The students will also pay a visit to a biotechnological firm and/or research institution.
Prerequisites
A basic knowledge of genetics, biochemistry and biotechnology.
Final Objectives
· Attitud lifelong learning
· Oral and written communication skills
· Thorough knowledge and implementation of the research and analytical instrumentarium of the genetic analyses
· Creativity and initiative in experimental set ups
· Manage, analyse and evaluate research results and formulatie conclusions
· Basic knowledge of side disciplines and implementation in research and production
· Set up of interdisciplinary research
Materials used
Syllabus
Textbooks and scientific papers; websites
Study costs
Cost: 10.0 EUR
± 10 Euro
Study guidance
Additional information can always be asked
Teaching Methods
Lectures; laboratory excercises and visit to research institution
Assessment
Theory: Written examination with open questions
Practicals: permanent evaluation and quotation of the reports
Lecturer(s)
T.: Kathy Messens
P: Els Palmans
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