Key words
Biotechnology, Molecular Genetics
Objectives
The biotechnology goes far by technical innovations towards improvements of the environment and the circumstances of live.
Topics
The DNA molecule is the molecular basis of genetics.
His complex structure and his organisation into chromosomes is the starting-point for the study of mutations.
The molecular basis of genetic engineering and the explanation of practical realizations.
Sequence analysis of proteins and nucleic acids in solution or on a carrier (automatisation).
Organic synthesis of proteins and nucleic acids in solution or on a carrier.
Constructions of recombinants : vector, clones, genomic library
Protein modificationIn the practical courses : separation of proteins, nucleic acids, plasmids.
Stability of plasmids.
Use of restriction enzymes.
Isolation of enzymes.
Immobilisation of cells and their use in bioreactors.
Prerequisites
Profound knowledge of enzymology, molecular biology and biochemistry.
Final Objectives
The student should be able to analyse and solve practical problems by linking them to the course curriculum.
An ability to interpret results and to state them in the right context is expected, together with the ability of detecting errors and fallacies.
Materials used
The book ‘Molecular Cell Biology’ edited by Lodish et all. (Freeman Company Edition).
Teacher’s course for practical and theoretical course.
Study costs
Cost: 67 EUR (€ 62 for the book and € 5 for the teacher course).
Study guidance
Possibility to consult the teacher after the lecture or by appointment.
Electronic: http://docent.hogent.be
Teaching Methods
Lectures and exercises.
Assessment
Oral examination for the theoretical course : 80 %
Permanent evaluation for de exercises : 20 %
A weighted average is used to compute the final score for a training item.
However, if a student gains a score of 7 or less on 20 on one of the different courses (parts of training items), he proves that his skill for certain subcompetences is insufficient. Consequently, one can turn from the arithmetical calculation of the final assignment of quotas of a training item and the new marks can be awarded on consensus.
Lecturer(s)
Raf Rogiers
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