Key words
molecular biotechnology, techniques for biochemical analysis, immunology
Objectives
acquire practical skills in the right follow up of procedures in molecular research, to reach reproducable accurate results and the representation of procedures, results, problems and conclusions on an adequate, well-organized and understandable way
Topics
Molecular biotechnology (50%)
- isolation procedures for DNA
- PCR
- agarose-electrophoresis
- transformation of E. coli with a GFP containing plasmid
- induction of the ß-galactosidase gen in E. coli
- theoretical exercices
- induction of callus on plant tissue
Techniques for biochemical analysis (30%)
- isolation procedures for proteins like GFP
- immobilisation of cells or proteins
- SDS-page and western-blotting
- use of fluorescence and luminescence measurements for the quality of biological samples
Immunology (17 %)
- immunodiffusion and immuno-electrophoresis
- bloodgroup determinations: ABO and Rh
- ELISA
- haemagglutination inhibition test
Prerequisites
Good theoretical knowledge of biochemistry, microbiology and genetics.
Practical experiences with biochemical analysis and microbiological handling.
Final Objectives
The student is capable in group to understand practical protocols, to preform them accurately and within a timelimit and to acquire reproducable results. He or she can interpret the acquired results, conclude about further procedures and make good decisions. The student can solve unexpected problems and can convince his collegues to solve the problems in an adequate way. The student is capable of writting a good report and/or give a spoken clear and well-grounded overview to his/her superior.
Materials used
teachers course
Study costs
10 euro
Study guidance
permanent during the practical sessions
Teaching Methods
practical exercices with support
Assessment
permanent evaluation during the exercices and based upon the report made after each experiment
Lecturer(s)
Katrien Strubbe
Ann Messens
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