Key words
Biochemistry, Enzymology, Biokatalysis
Objectives
Close examination of the actions and the properties of free and immobilized enzymes in view of their applications in enzymatic analysis and industrial processes.
Topics
The structure of several groups of biocatalysts, the kinetics and the diverse forms of inhibition are discussed.
Because enzymes catalyse unique reactions with high selectivity and under mild conditions, their industrial applications are emphasized. The importance of enzymes in a number of industries is highlighted (e.g. the pulp- and paper production, the feed and food industry, in crop protection and the pharmaceutical industry). The industrial preparation of enzymes is also discussed.
The kinetic parameters and the inhibition of the enzymatic reactions are determined in the practical courses. The exercises also deal with enzyme purification and enzymatic detection (e.g. zymograms).
Prerequisites
Knowledge of the principles of organic chemistry and biochemistry
Final Objectives
General scientific competences (AWC1, AWC2 and AWC3)
- Demonstrating an understanding of the mechanism of enzyme action and of the kinetics of enzyme-catalysed reactions.
- Learning to adopt a critical, scientific and problem-solving attitude
- Learning to practise numeracy and scientific reporting
General competences (AC1)
- Developing group working skills
Specific competences (SC1, SC2 en SC6)
- Developing skills concerning working on micro-scale and dealing with expensive substrates and enzymes
Materials used
Teacher’s course for practical and theoretical course.
Study costs
Cost: 7.0 euro
Study guidance
Students are guided intensively during the practical sessions
Teaching Methods
Lectures and lab exercises
Assessment
Oral examination of the theoretical courses : 66,66 %
Permanent evaluation of the practical courses and quoted lab report: 33,33 %
A weighted average is used to compute the final score for a training item.
However, if a student gains a score of 7 or less on 20 on one of the different courses (parts of training items), he proves that his skill for certain subcompetences is insufficient. Consequently, one can turn from the arithmetical calculation of the final assignment of quotas of a training item and the new marks can be awarded on consensus.
Lecturer(s)
Ingeborg Stals
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