Key words
Methods of biochemical analysis
Objectives
Quality control needs accurate methods for rapid analysis. The identification and the quantification of specific biomolecules are possible by use of a great diversity of biochemical methods for analyzing even complex samples.
Topics
- The use of agarose and p.a.a. gels in diverse electrophoretic separation methods for proteins and nucleic acids.
- Detection of biomolecules by fluorescent dyes.
- Immunological methods: diffusion, electrophoresis, ELISA
- The detection of microbiological contamination by looking for ATP, lipides, enzymes and toxines
- DNA-technology: in situ hybridisation, blotting, PCR and deduced methods.
- Comparision of the classical microbiological methods and the new DBA-based methods
Prerequisites
Basic knowledge of biochemistry, enzymology and microbiology.
Final Objectives
Materials used
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Teacher's course - other textbook(s) - school library materials
Study costs
Study guidance
Teaching Methods
Lectures - demonstrations
Assessment
Theoretical course: oral examination
Practical course: evaluation during course - report of the results.
However, if a student gains a score of 7 or less on 20 on one of the different courses (parts of training items), he proves that his skill for certain subcompetencies is insufficient. Consequently, one can turn from the arithmetical calculation of the final assignment of quotas of a training item and the new marks can be awarded on consensus. Of course the examiners can judge that the arithmetic regulations mentioned in the study index card can also be used for 7 or less. For each deviation a detailed motivation ought to be drawn up. In that case one should point out that the skill for this subcompetency is proven to be insufficient, if the student didn’t pass the partim that is considered to be important for certain subcompetencies.
Lecturer(s)
Raphaël ROGIERS
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